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MetaSystems inc isis 3 situ imaging system) software
Isis 3 Situ Imaging System) Software, supplied by MetaSystems inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isis+3+software/isis+3+software/pm33070101-56-18-24
Average 90 stars, based on 1 article reviews
isis 3 situ imaging system) software - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Sexing river buffalo (Bubalus bubalis L.), sheep (Ovis aries L.), goat (Capra hircus L.), and cattle spermatozoa by double color FISH using bovine (Bos taurus L.) X- and Y-painting probes.
Article Snippet: River buffalo, sheep, and goat spermatozoa were cross-hybridized using double color fluorescence in situ hybridization (FISH) with bovine Xcenand Y-chromosome painting probes, prepared by DOP-PCR of laser-microdissected-catapulted chromosomes, to investigate the possibility of using bovine probes for sexing sperm of other members of the family Bovidae.. Before sperm analysis, the probes were hybridized on metaphase chromosomes of each species, as control.. Frozen-thawed spermatozoa of cattle, river buffalo, sheep, and goat were decondensed in suspension with 5 mM DTT.

Article Title: Centric fusion polymorphism in captive animals of family Bovidae
Article Snippet: Chromosomes of 228 captive specimens of the family Bovidae have been investigated.. The examined animals were classified into the subfamilies Aepycerotinae, Reduncinae, Antilopinae, Alcelaphinae, Hippotraginae and Bovinae.. Polymorphism for one fusion was identified in the species: Aepyceros melampus, 2n = 59–60; Redunca fulvorufula, 2n = 56–57; Kobus e. ellipsiprymnus, 2n = 50– 52; Kobus e. defassa, 2n = 52–54 and Syncerus c. nanus, 2n = 54–55.

Article Title: Phylogenomic study of spiral-horned antelope by cross-species chromosome painting.
Article Snippet: Chromosomal homologies have been established between cattle (Bos taurus, 2n = 60) and eight species of spiralhorned antelope, Tribe Tragelaphini: Nyala (Tragelaphus angasii, 2n = 55S/569), Lesser kudu (T. imberbis, 2n = 38S,9), Bongo (T. eurycerus, 2n = 33S/349), Bushbuck (T. scriptus, 2n = 33S/349), Greater kudu (T. strepsiceros, 2n = 31S/329), Sitatunga (T. spekei, 2n = 30S,9) Derby eland (Taurotragus derbianus 2n = 31S/329) and Common eland (T. oryx 2n = 31S/329).. Chromosomes involved in centric fusions in these species were identified using a complete set of cattle painting probes generated by laser microdissection.. Our data support the monophyly of Tragelaphini and a clade comprising T. scriptus, T. spekei, T. euryceros and the eland species T. oryx and T. derbianus, findings that are largely in agreement with sequence-based molecular phylogenies.

Article Title: Isolation and physical localization of new chromosome-specific centromeric repeats in farm animals
Article Snippet: Probe signals on metaphase spreads were visualized under an Olympus BX60 fluorescent microscope. .. Digital images were captured using the ISIS 3 software (MetaSystems, Altlussheim, Germany). ..

Article Title: Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas.
Article Snippet: Results.. The examined tumors exhibited five and up to 47 DNA copy number alterations (CNAs).. Nineteen of these showed a gain of chromosome band 7p12.

Article Title: Identification of a new reciprocal translocation in an AI bull by synaptonemal complex analysis, followed by chromosome painting.
Article Snippet: tion of the translocation.. Until now the number of different reciprocal translocations found in cattle is rather low – only fifteen cases were reported (for review see Switonski and Stranzinger, 1998; De Lorenzi et al., 2007; Ducos et al., 2008).. It is well known that fertility of bulls carrying a reciprocal translocation is altered.

Article Title: The utility of chromosome microdissection in clinical cytogenetics: a new reciprocal translocation in sheep.
Article Snippet: Local sheep breeders and scientists in Italy cooperate and conduct research on the genetic improvement of autochthonous genetic types (AGTs) by various approaches, including a cytogenetic breeding selection since 2011.. The Laticauda sheep (Ovis aries, 2n = 54) breed is one of the AGTs reared in the Campania region (southern Italy).. Performing cytogenetic analyses, we have detected and described a novel reciprocal translocation in a Laticauda sheep identified as 54,XX t(18; 23)(q14;q26).

Microscopy:

Article Title: Phylogenomic study of spiral-horned antelope by cross-species chromosome painting.
Article Snippet: Chromosomal homologies have been established between cattle (Bos taurus, 2n = 60) and eight species of spiralhorned antelope, Tribe Tragelaphini: Nyala (Tragelaphus angasii, 2n = 55S/569), Lesser kudu (T. imberbis, 2n = 38S,9), Bongo (T. eurycerus, 2n = 33S/349), Bushbuck (T. scriptus, 2n = 33S/349), Greater kudu (T. strepsiceros, 2n = 31S/329), Sitatunga (T. spekei, 2n = 30S,9) Derby eland (Taurotragus derbianus 2n = 31S/329) and Common eland (T. oryx 2n = 31S/329).. Chromosomes involved in centric fusions in these species were identified using a complete set of cattle painting probes generated by laser microdissection.. Our data support the monophyly of Tragelaphini and a clade comprising T. scriptus, T. spekei, T. euryceros and the eland species T. oryx and T. derbianus, findings that are largely in agreement with sequence-based molecular phylogenies.

Article Title: Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas.
Article Snippet: Results.. The examined tumors exhibited five and up to 47 DNA copy number alterations (CNAs).. Nineteen of these showed a gain of chromosome band 7p12.

Article Title: Cytotypes of Kirk's dik-dik (Madoqua kirkii,Bovidae) show multiple tandem fusions.
Article Snippet: Madoqua kirkii , a miniature African antelope, is noted for extensive chromosomal variation that has been categorized in four distinct cytotypes (A–D).. In this investigation, we analyzed the A cytotype (2n = 46, FN = 48) using a suite of molecular cytogenetic approaches that entailed (i) whole chromosome and subchromosomal painting by fluorescence in situ hybridization (FISH), (ii) the study of Madoqua centromeric-specific DNA derived from pooled DNA obtained from the centromeric regions of the acrocentric chromosomes, and (iii) DNA from the telomere:centromere junctions of tandemly fused chromosomes.. DNA from these sources was used to probe for the persistence of interstitial satellite DNA and residual centromeric sequences in the tandem and centric fusion junctions by PCR and FISH.

Fluorescence:

Article Title: Genomic gain of the epidermal growth factor receptor harboring band 7p12 is part of a complex pattern of genomic imbalances in oral squamous cell carcinomas.
Article Snippet: Results.. The examined tumors exhibited five and up to 47 DNA copy number alterations (CNAs).. Nineteen of these showed a gain of chromosome band 7p12.

Hybridization:

Article Title: Cytotypes of Kirk's dik-dik (Madoqua kirkii,Bovidae) show multiple tandem fusions.
Article Snippet: Madoqua kirkii , a miniature African antelope, is noted for extensive chromosomal variation that has been categorized in four distinct cytotypes (A–D).. In this investigation, we analyzed the A cytotype (2n = 46, FN = 48) using a suite of molecular cytogenetic approaches that entailed (i) whole chromosome and subchromosomal painting by fluorescence in situ hybridization (FISH), (ii) the study of Madoqua centromeric-specific DNA derived from pooled DNA obtained from the centromeric regions of the acrocentric chromosomes, and (iii) DNA from the telomere:centromere junctions of tandemly fused chromosomes.. DNA from these sources was used to probe for the persistence of interstitial satellite DNA and residual centromeric sequences in the tandem and centric fusion junctions by PCR and FISH.



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